Document Details
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Document Type |
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Article In Journal |
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Document Title |
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A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis |
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Document Language |
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English |
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Abstract |
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Amplicon (targeted) sequencing by massively parallel sequencing (PCR-MPS) is a potential method for use in forensic DNA analyses. In this application, PCR-MPS may supplement or replace other instrumental analysis methods such as capillary electrophoresis and Sanger sequencing for STR and mitochondrial DNA typing, respectively. PCR-MPS also may enable the expansion of forensic DNA analysis methods to include new marker systems such as single nucleotide polymorphisms (SNPs) and insertion/deletions (indels) that currently are assayable using various instrumental analysis methods including microarray and quantitative PCR. Acceptance of PCR-MPS as a forensic method will depend in part upon developing protocols and criteria that define the limitations of a method, including a defensible analytical threshold or method detection limit. This paper describes an approach to establish objective analytical thresholds suitable for multiplexed PCR-MPS methods. A definition is proposed for PCR-MPS method background noise, and an analytical threshold based on background noise is described. |
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ISSN |
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1932-6203 |
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Journal Name |
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PLOS ONE |
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Volume |
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12 |
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Issue Number |
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5 |
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Publishing Year |
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1438 AH
2017 AD |
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Article Type |
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Article |
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Added Date |
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Wednesday, June 26, 2019 |
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Researchers
| Brian Young | Young, Brian | Researcher | Doctorate | brian@nichevision.com |
| Jonathan L. King | King, Jonathan L. | Researcher | Doctorate | |
| Bruce Budowle | Budowle, Bruce | Researcher | Doctorate | |
| Luigi Armogida | Armogida, Luigi | Researcher | Doctorate | |
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